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Leading researchers and experimentalists update the field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and fluorescent in situ hybridization. Protocols for chromosome sorting and 5-6 color analysis are included as advanced methods. The techniques in this volume take advantage of the new instrumentation and probes that have recently been developed to advance the field of flow cytometry and improve standard methods.

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Recent advances in our understanding of the biochemical and molecular mechanisms of cell growth and control have led to sensational breakthroughs in the diagnosis and treatment of proliferative and neoplastic diseases. As a consequence, cell growth and regulation has become a subject of vital interest to basic researchers and clinicians alike. Never has the need for authoritative reviews such as this been greater.

Comprising contributions by an international team of scientists at the forefront of one of today's liveliest areas of biomedical research, this book reports on the state-of-the-science in cell growth and regulation from the conceptual, experimental, and clinical perspective. Written at a level that will appeal to professionals as well as graduate and medical students, it explores a wide range of related topics, with an emphasis on the identification of checkpoints and restriction points and the mechanisms of proliferation competency and cell cycle progression, including:
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provides a state-of-the-art review of medical mycology compiled by the leading researchers in the field; presents a selection of chapters covering immunological aspects of infection; captures the excitement of recently completed genome projects and their impact on the understanding of fungal biology and virulence; examines the recent advances in the development of fungal vaccines; features an in-depth analysis of the host-pathogen relationship.

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